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Extract DNA samples and removing contamination

$30-250 USD

Completed
Posted about 6 years ago

$30-250 USD

Paid on delivery
My research is on detection of genetic diversity in rice using RAPD markers. I have 40 samples of rice seeds. I have extracted the genomic DNA from all the 40 samples and I found there was RNA contamination. I dint use any RNase during the extraction process. So now I have to do RNase A treatment post genomic DNA extraction. The RNase A in my lab is in the lyophilized powder form and I made a 10mg/ml RNase by diluting 0.01g of RNase A in 1 ml of autoclaved miliQ water. Later I treated my genomic DNA (50 µl) with 1 µl of RNase A and incubated it at 37 Celsius for 1 hour. Upon treatment with RNase A, I found a lot of my genomic DNA got degraded. However, my subsequent step is PCR. I am not sure if the very faint level of my genomic DNA is sufficient for my next step which is PCR. i need help With the procedures to get this right to have sufficient DNA without contamination for my NeXT step
Project ID: 16463697

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5 proposals
Remote project
Active 6 yrs ago

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Hi there, I can help you with your genomic DNA extraction protocol. After my masters in the UK, I am currently working as a researcher in Germany (molecular biology/Bioinformatics) and I have lot of experience in molecular biology wet lab techniques along with in-silico sequence analysis. Do let me know if interested Regards
$222 USD in 3 days
4.9 (7 reviews)
3.8
3.8
5 freelancers are bidding on average $225 USD for this job
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Hello, I am a professional researcher. My educational background is pharmaceutics. I have read your project description and I understand what you want in this project. I am looking forward to work on this project. Kindly contact me to discuss the details. Best Regard, Ahmad Zia
$190 USD in 4 days
5.0 (57 reviews)
5.6
5.6
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Hi I am PhD in medical biotechnology and microbiology. I have 7 years of research and writing experience and have worked on PhD level thesis projects, published papers in peer reviewed journals and have also worked with several companies on creating research based reports, books, blogs, essays, white papers, patents, SEO articles, product descriptions and formulating novel products. I have excellent academic writing skills and statistical analysis skills in R , spss, excel to complete any assignment in high quality. Looking forward to discussion, Best Regards, Suyash
$350 USD in 7 days
4.9 (26 reviews)
4.9
4.9
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Hello, My brother has PhD in Agronomy and Plant Genetics, with 15+ years of working experience as researcher and professor in the UK. And recent research was 'Modeling and genome of canopy photosynthesis in Rice'. Let me know if you would be interested in our services and I'll be glad to assist you. Best regards!
$155 USD in 7 days
5.0 (1 review)
3.1
3.1
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Hello, i have read the details provided..please contact me to discuss more on the project deadline and some other few things
$210 USD in 3 days
4.9 (2 reviews)
2.3
2.3

About the client

Flag of NORWAY
fosnavåg, Norway
5.0
3
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Member since Mar 16, 2014

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